Exploring Mechanisms of Neuroserpin Regulation in Alzheimer's Disease
Researchers:
Funding year(s):
2010 - 2011
Funding to date:
$100,000
Understanding the up-regulation of Neurosperpin in Alzheimer’s disease brain and isolating a potential therapeutic inhibitor of its action; a continuation.
Specific aim #1 for the project is to determine what is responsible for the up-regulation of neuroserpin in the Alzheimer’s disease brain?
Specific aim #2for the project is to use a sensitive high throughput assay screen for inhibitors of Neuroserpin to:
- Screen commercially available libraries and selected chemical compounds.
- b. Use medicinal chemistry to optimize and design new molecules based on the structures of identified hit candidate molecules (above) and crystal structure of neuroserpin.
- Confirmation of screening “hits”: rule out false positives, do dose response curves and counter-screens to identify “leads”.
- Test for “hit” interaction with neuroserpin, possible mechanism of action and potential toxicity in hippocampal neuronal cell cultures.
