Single-cell analyses have identified multiple cell subsets in the central nervous system (CNS). However, our understanding of the mechanisms that mediate CNS cell-cell communication in homeostasis and pathology is limited. A deep understanding of these cell-cell interactions and their perturbations is needed to define mechanisms associated with CNS aging and Alzheimer’s disease (AD) pathogenesis. We propose to use novel and complementary technologies to comprehensively investigate regulatory cell-cell interactions in the CNS, their dysregulation in AD and their potential as therapeutic targets.
