Cure Alzheimer's Fund Cure Alzheimer's Fund
Through a grant provided by Cure Alzheimer’s Fund, Drs. Rudy Tanzi and Doo Yeon Kim created Alzheimer’s in a Dish which features colonies of genetically manipulated human brain cells that grow in three dimensions in a specialized gel. Once added to the medium, the cells begin to display the two most salient hallmarks of Alzheimer’s disease: plaques and tangles. Forming around and between the cells, the microscopic plaques consist of cast-off protein fragments called amyloid-beta while the tiny tau tangles develop just as in the diseased brain. Plaques and tangles are the same defects that Alois Alzheimer observed more than a century ago as he examined the autopsied brains of patients who suffered from the disease. Scientists were not able to create both components of the pathology simultaneously in the lab until the creation of Alzheimer’s in a Dish. Now, with this discovery, scientists can create the pathology in the lab and conduct tests against the development of the disease — including reviews of existing drugs to determine possibility efficacy.

 

 

The following is the transcript from an article published on the Harvard Medical School website, October 14, 2014 by Sue McGreevey.

 

“An innovative laboratory culture system has succeeded, for the first time, in reproducing the full course of events underlying the development of Alzheimer’s disease. Using the system they developed, Harvard Medical School investigators at Massachusetts General Hospital now provide the first clear evidence supporting the hypothesis that deposition of beta-amyloid plaques in the brain is the first step in a cascade leading to the devastating neurodegenerative disease. They also identify the essential role in that process of an enzyme, whose inhibition could be a therapeutic target.”

Their findings are published in Nature.

 

“Originally put forth in the mid-1980s, the amyloid hypothesis maintained that beta-amyloid deposits in the brain set off all subsequent events-the neurofibrillary tangles that choke the insides of neurons, neuronal cell death and inflammation leading to a vicious cycle of massive cell death,” said Rudolph Tanzi, Joseph P. and Rose F. Kennedy Professor of Child Neurology and Mental Retardation at Harvard Medical School and Massachusetts General Hospital. He is director of the hospital’s Genetics and Aging Research Unit and co-senior author of the paper. “One of the biggest questions since then has been whether beta-amyloid actually triggers the formation of the tangles that kill neurons. In this new system that we call “Alzheimer’s-in-a-dish,” we’ve been able to show for the first time that amyloid deposition is sufficient to lead to tangles and subsequent cell death.”

While mouse models of Alzheimer’s disease that express the gene variants causing the inherited early-onset form of the disease do develop amyloid plaques in their brains and memory deficits, the neurofibrillary tangles that cause most of the damage do not appear. Other models succeed in producing tangles but not plaques. Cultured neurons from human patients with Alzheimer’s exhibit elevated levels of the toxic form of amyloid found in plaques and the abnormal version of the tau protein that makes up tangles but they do not exhibit actual plaques and tangles.

Genetics and Aging Research Unit investigator Doo Yeon Kim, Harvard Medical School assistant professor of neurology at Massachusetts General Hospital and co-senior author of the Nature paper, realized that the liquid two-dimensional systems usually used to grow cultured cells poorly represent the gelatinous three-dimensional environment within the brain. Instead, the Massachusetts General Hospital team used a gel-based, three-dimensional culture system to grow human neural stem cells that carried variants in two genes-which produce the amyloid precursor protein and presenilin 1-known to underlie early-onset familial Alzheimer’s disease. Both of those genes were co-discovered in Tanzi’s laboratory.

After growing for six weeks, cells with the early-onset familial Alzheimer’s disease variant were found to have significant increases in both the typical form of beta amyloid and the toxic form associated with Alzheimer’s. The variant cells also contained the neurofibrillary tangles that choke the inside of nerve cells, causing cell death. Blocking steps known to be essential for the formation of amyloid plaques also prevented the formation of the tangles, confirming amyloid’s role in initiating the process. The version of tau found in tangles is characterized by the presence of excess phosphate molecules; when the team investigated possible ways of blocking tau production, they found that inhibiting the action of an enzyme called GSK3-beta-known to phosphorylate tau in human neurons-prevented the formation of tau aggregates and tangles even in the presence of abundant beta-amyloid and amyloid plaques.

“This new system-which can be adapted to other neurodegenerative disorders-should revolutionize drug discovery in terms of speed, costs and physiologic relevance to disease,” Tanzi said.

“Testing drugs in mouse models that typically have brain deposits of either plaques or tangles, but not both, takes more than a year and is very costly. With our three-dimensional model that recapitulates both plaques and tangles, we now can screen hundreds of thousands of drugs in a matter of months without using animals in a system that is considerably more relevant to the events occurring in the brains of Alzheimer’s patients.”

The study was supported by a grant from the Cure Alzheimer’s Fund and by National Institutes of Health grants 5P01AG15379 and 5R37MH060009.

Since the initial development of Alzheimer’s in a Dish, enhancements have been made that now provide for the development of tau tangles, as well as inflammation, in the environment of a petri dish; the three primary components of the pathology of Alzheimer’s disease. The formula for the medium is available to scientists for their own use in the investigation of Alzheimer’s disease.

Topics of Interest